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Human Protein Atlas immunohistochemical images
Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) <t>Immunohistochemical</t> validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.
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1) Product Images from "LATS2 expression differences in lung adenocarcinoma and lung squamous cell carcinoma analyzed using bioinformatics and experimental approaches"

Article Title: LATS2 expression differences in lung adenocarcinoma and lung squamous cell carcinoma analyzed using bioinformatics and experimental approaches

Journal: Oncology Letters

doi: 10.3892/ol.2026.15671

Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) Immunohistochemical validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.
Figure Legend Snippet: Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) Immunohistochemical validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.

Techniques Used: Expressing, Quantitative Proteomics, Immunohistochemical staining, Biomarker Discovery, RNA Sequencing

Related Articles

Immunohistochemistry:

Article Title: Identification of immune-related gene signature for predicting prognosis of glioblastoma patients
Article Snippet: .. IHC images for the core genes were from the Human Protein Atlas (HPA), with selection criteria including (I) GBM or normal brain tissue; (II) high-specificity antibodies validated by HPA; and (III) exclusion of blurred or non-specific staining images. ..

Article Title: TRIM21-mediated degradation of HILPDA overcomes anti-PD-1 immunotherapy resistance in breast cancer by limiting PD-L1 palmitoylation
Article Snippet: .. We next queried The Human Protein Atlas (HPA) for breast/breast-cancer specimens and incorporated IRB-approved clinical paraffin sections from our center to obtain IHC images/annotations for TRIM21 and HILPDA. ..

Article Title: Integrated single-cell and spatial transcriptomics reveal the differentiation drivers of gastric epithelial lineage progression
Article Snippet: .. Protein expression and IHC images were obtained from the Human Protein Atlas. ..

Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma
Article Snippet: .. All IHC images, patient metadata (including age, sex, and patient ID), and staining data were retrieved from the publicly available Human Protein Atlas resource. ..

Article Title: Integrated single-cell and spatial transcriptomics reveal the differentiation drivers of gastric epithelial lineage progression
Article Snippet: .. Consistent with IHC images from the Human Protein Atlas confirmed that UPP1 was barely detectable in normal gastric tissues but strongly expressed in gastric adenocarcinoma specimens, particularly in the cytoplasmic and membranous compartments of tumor cells ( , left panel). ..

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..

Article Title: TRIM21-mediated degradation of HILPDA overcomes anti-PD-1 immunotherapy resistance in breast cancer by limiting PD-L1 palmitoylation.
Article Snippet: .. We next queried The Human Protein Atlas (HPA) for breast/breast-cancer specimens and incorporated IRB-approved clinical paraffin sections from our center to obtain IHC images/annotations for TRIM21 and HILPDA. ..

Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma.
Article Snippet: .. All IHC images, patient metadata (including age, sex, and patient ID), and staining data were retrieved from the publicly available Human Protein Atlas resource. ..

Selection:

Article Title: Identification of immune-related gene signature for predicting prognosis of glioblastoma patients
Article Snippet: .. IHC images for the core genes were from the Human Protein Atlas (HPA), with selection criteria including (I) GBM or normal brain tissue; (II) high-specificity antibodies validated by HPA; and (III) exclusion of blurred or non-specific staining images. ..

Staining:

Article Title: Identification of immune-related gene signature for predicting prognosis of glioblastoma patients
Article Snippet: .. IHC images for the core genes were from the Human Protein Atlas (HPA), with selection criteria including (I) GBM or normal brain tissue; (II) high-specificity antibodies validated by HPA; and (III) exclusion of blurred or non-specific staining images. ..

Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma
Article Snippet: .. All IHC images, patient metadata (including age, sex, and patient ID), and staining data were retrieved from the publicly available Human Protein Atlas resource. ..

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..

Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma.
Article Snippet: .. All IHC images, patient metadata (including age, sex, and patient ID), and staining data were retrieved from the publicly available Human Protein Atlas resource. ..

Expressing:

Article Title: Integrated single-cell and spatial transcriptomics reveal the differentiation drivers of gastric epithelial lineage progression
Article Snippet: .. Protein expression and IHC images were obtained from the Human Protein Atlas. ..

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..

RNA Sequencing:

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..

Functional Assay:

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..

Biomarker Discovery:

Article Title: Celastrol induces ferroptosis in ccRCC through TEF-Driven ACOX2 upregulation and metabolic reprogramming.
Article Snippet: .. ACOX2 is identified as a key mediator of celastrol-induced cytotoxicity in ccRCC cells. (A–B) RNA sequencing of 786-O and 769-P cells treated with celastrol or DMSO identifying differentially expressed genes; (C) GO enrichment analysis of celastrol target genes from CTD showing significant enrichment in metabolic pathways; (D) Venn diagram integrating metabolic pathway–related genes from GeneCards with differentially expressed genes in ccRCC cells after CEL treatment (CEL DEGs) and DEGs from TCGA KIRC cohort (KIRC DEGs), yielding 27 overlapping candidates; (E) Cytoscape-based network analysis of the 27 candidate genes revealing functional clusters including lipid metabolic regulation; (F) Prognostic analysis of 24 candidate genes (three not available in GSCA) using TCGA-KIRC data showing associations with OS and PFS; (G–H) Expression analysis demonstrating reduced ACOX2 expression in ccRCC tissues compared with normal kidney tissues, confirmed in paired samples; (I) Kaplan–Meier survival curves showing higher ACOX2 expression associated with improved OS and PFS in KIRC patients; (J) Human Protein Atlas IHC images showing stronger ACOX2 staining in normal renal tubules compared with ccRCC tissues; (K) GO enrichment analysis of ACOX2associated genes in the KIRC dataset demonstrating predominant involvement in metabolic processes. require complementary biochemical validation. ..



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Human Protein Atlas immunohistochemical images
Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) <t>Immunohistochemical</t> validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.
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Human Protein Atlas immunostaining images
Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Human Protein Atlas immunohistochemical ihc images depicting eno1
Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Human Protein Atlas 363 immunohistochemistry ihc images
Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Human Protein Atlas immunohistochemistry ihc staining 764 images
Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Akoya Biosciences assays opal polaris 7 color manual ihc kit akoya biosciences cat
Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the <t>immunostaining</t> images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.
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Integrated in silico and experimental analysis of transferrin receptor 1 <t>(TfR1</t> / TFRC) expression in gliomas and representative cell lines. Box-plot summary of TFRC mRNA expression obtained from GEPIA (Gene Expression Profiling Interactive Analysis) based on tumor and normal samples from the TCGA and the GTEx databases (accessed March 2025). ( A ) Comparison of TFRC expression in high-grade gliomas (GBM) vs. low-grade glioma (LGG) (n indicated on each plot). ( B ) TFRC expression stratified by canonical GBM molecular subtype (classical, mesenchymal, neural, proneural). Boxes represent the interquartile range, horizontal lines the median, whiskers extend to 1.5×IQR, and individual data points are overlaid. Brackets with asterisks denote statistically significant pairwise differences (see Methods for statistical test). ( C ) TfR1 expression levels in GBM cell lines and HEK293 (non-tumor control). Data taken and adapted from the Human Protein Atlas. ( D ) Representative flow-cytometry histograms of surface TfR1 staining in U87MG, T98G, MO59K and HEK293 cells. Traces correspond to unstained control (red), secondary-only control (anti-mouse IgG–AF647; Orange) and specific anti-CD71 primary staining followed by anti-mouse-AF647 secondary (blue). The horizontal bracket on each histogram indicates the gate used to define TfR1-positive events. ( E ) gMFI of the CD71 signal (mean ± SD; n = biological replicates indicated in Methods), and ( F ) percentage of TfR1-positive cells. Data were normalized to appropriate controls. Statistical comparisons were performed as described in Methods (* p < 0.05 ).
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Integrated in silico and experimental analysis of transferrin receptor 1 <t>(TfR1</t> / TFRC) expression in gliomas and representative cell lines. Box-plot summary of TFRC mRNA expression obtained from GEPIA (Gene Expression Profiling Interactive Analysis) based on tumor and normal samples from the TCGA and the GTEx databases (accessed March 2025). ( A ) Comparison of TFRC expression in high-grade gliomas (GBM) vs. low-grade glioma (LGG) (n indicated on each plot). ( B ) TFRC expression stratified by canonical GBM molecular subtype (classical, mesenchymal, neural, proneural). Boxes represent the interquartile range, horizontal lines the median, whiskers extend to 1.5×IQR, and individual data points are overlaid. Brackets with asterisks denote statistically significant pairwise differences (see Methods for statistical test). ( C ) TfR1 expression levels in GBM cell lines and HEK293 (non-tumor control). Data taken and adapted from the Human Protein Atlas. ( D ) Representative flow-cytometry histograms of surface TfR1 staining in U87MG, T98G, MO59K and HEK293 cells. Traces correspond to unstained control (red), secondary-only control (anti-mouse IgG–AF647; Orange) and specific anti-CD71 primary staining followed by anti-mouse-AF647 secondary (blue). The horizontal bracket on each histogram indicates the gate used to define TfR1-positive events. ( E ) gMFI of the CD71 signal (mean ± SD; n = biological replicates indicated in Methods), and ( F ) percentage of TfR1-positive cells. Data were normalized to appropriate controls. Statistical comparisons were performed as described in Methods (* p < 0.05 ).
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Image Search Results


Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) Immunohistochemical validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.

Journal: Oncology Letters

Article Title: LATS2 expression differences in lung adenocarcinoma and lung squamous cell carcinoma analyzed using bioinformatics and experimental approaches

doi: 10.3892/ol.2026.15671

Figure Lengend Snippet: Comprehensive expression analysis of LATS2 in LUAD and LUSC. (A) Expression levels of LATS2 in LUAD and LUSC analyzed using the Gene Set Cancer Analysis platform. (B) Comparative analysis of LATS2 expression between tumor and normal tissues in LUAD using independent sample t-test and paired sample t-test. (C) Comparative analysis of LATS2 expression between tumor and normal tissues in LUSC using independent sample t-test and paired sample t-test. (D) Differential expression of LATS2 between LUAD and LUSC tumor tissues. (E) Immunohistochemical validation of LATS2 expression in LUAD, LUSC and normal lung tissues using the Human Protein Atlas database (antibody ID: 039191). All the data are presented as mean ± SD. ***P<0.001. LATS2, large tumor suppressor kinase 2; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; TPM, transcripts per million; RSEM, RNA-seq by expectation-maximization.

Article Snippet: Immunohistochemical images obtained from the Human Protein Atlas database indicated that the proportion of LATS2-positive cells was markedly higher in normal lung tissues compared with LUAD and LUSC tumor tissues ( ).

Techniques: Expressing, Quantitative Proteomics, Immunohistochemical staining, Biomarker Discovery, RNA Sequencing

Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the immunostaining images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.

Journal: iScience

Article Title: ExIR enables prioritizing driver and biomarker genes from omics data in a reference free manner

doi: 10.1016/j.isci.2026.116303

Figure Lengend Snippet: Differential immunoreactivity of known and novel ExIR-predicted LUAD biomarkers between normal and cancer samples (A–E) Immunohistochemical (IHC) data from the Human Protein Atlas database in LUAD and normal lung tissue for top five known LUAD biomarkers. (A) SFTPC–ExIR rank #1, LUAD (negative intensity; patient ID: 1847) and normal pneumocytes (quantity: 75%–25%; strong intensity; patient ID: 2268). (B) SPP1—ExIR rank #117, LUAD (quantity: >75%; moderate intensity; patient ID: 537) and normal pneumocytes (not detected; patient ID: 2268). (C) CBLC—ExIR rank #140, LUAD (quantity: >75%; moderate intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2417). (D) MDK—ExIR rank #247, LUAD (quantity: 75%–25%; strong intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2222). (E) MRC1 – ExIR rank #471, LUAD (undetected; patient ID: 1932) and normal macrophages (quantity: 75%–25%; strong intensity; patient ID: 2208). (F–I) IHC data of top five ExIR-predicted LUAD biomarkers (excluding SFTPC rank #1 already in a). (F) AGER – ExIR rank #2, LUAD (not detected; patient ID: 3144) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 4840). (G) EMP2 – ExIR rank #3, LUAD (not detected; patient ID: 1847) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2101). (H) CAV1 – ExIR rank #4, LUAD (not detected; patient ID: 1249) and normal pneumocytes (quantity: >75%; strong intensity; patient ID: 2208). i, RTKN2 – ExIR rank 5, LUAD (not detected; patient ID: 3003) and normal pneumocytes (quantity: <25%; moderate intensity; patient ID: 2268).(J–N) IHC data of top six ExIR-predicted LUAD up-regulated biomarkers (excluding FAM83A rank #2, for which no IHC data was available). (J) PYCR1 – ExIR rank #1, LUAD (quantity: >75%; moderate intensity; patient ID: 2777) and normal pneumocytes (not detected; patient ID: 2208). (K) TOP2A – ExIR rank #3, LUAD (quantity: 75%–25%; strong intensity; patient ID: 3003) and normal pneumocytes (quantity: 75%–25%; weak intensity; patient ID: 2101). (L) MMP11 – ExIR rank #4, LUAD (quantity: >75%; weak intensity; patient ID: 1847) and normal pneumocytes (not detected; patient ID: 2438). (M) TEDC2– ExIR rank 5, LUAD (quantity: 75%–25%; moderate intensity; patient ID: 4208) and normal pneumocytes (not detected; patient ID: 1470). (N) IQGAP3– ExIR rank 6, LUAD (quantity: >75%; strong intensity; patient ID: 3048) and normal pneumocytes (not detected; patient ID: 1470). Per the Human Protein Atlas database usage guidelines, the link to the immunostaining images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend. Ab: antibody; LUAD: lung adenocarcinoma.

Article Snippet: Per the Human Protein Atlas database usage guidelines, the link to the immunostaining images of all of the selected proteins in normal pneumocytes and LUAD samples are included as hyperlinks within the figure legend.

Techniques: Immunohistochemical staining, Immunostaining

Integrated in silico and experimental analysis of transferrin receptor 1 (TfR1 / TFRC) expression in gliomas and representative cell lines. Box-plot summary of TFRC mRNA expression obtained from GEPIA (Gene Expression Profiling Interactive Analysis) based on tumor and normal samples from the TCGA and the GTEx databases (accessed March 2025). ( A ) Comparison of TFRC expression in high-grade gliomas (GBM) vs. low-grade glioma (LGG) (n indicated on each plot). ( B ) TFRC expression stratified by canonical GBM molecular subtype (classical, mesenchymal, neural, proneural). Boxes represent the interquartile range, horizontal lines the median, whiskers extend to 1.5×IQR, and individual data points are overlaid. Brackets with asterisks denote statistically significant pairwise differences (see Methods for statistical test). ( C ) TfR1 expression levels in GBM cell lines and HEK293 (non-tumor control). Data taken and adapted from the Human Protein Atlas. ( D ) Representative flow-cytometry histograms of surface TfR1 staining in U87MG, T98G, MO59K and HEK293 cells. Traces correspond to unstained control (red), secondary-only control (anti-mouse IgG–AF647; Orange) and specific anti-CD71 primary staining followed by anti-mouse-AF647 secondary (blue). The horizontal bracket on each histogram indicates the gate used to define TfR1-positive events. ( E ) gMFI of the CD71 signal (mean ± SD; n = biological replicates indicated in Methods), and ( F ) percentage of TfR1-positive cells. Data were normalized to appropriate controls. Statistical comparisons were performed as described in Methods (* p < 0.05 ).

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: Integrated in silico and experimental analysis of transferrin receptor 1 (TfR1 / TFRC) expression in gliomas and representative cell lines. Box-plot summary of TFRC mRNA expression obtained from GEPIA (Gene Expression Profiling Interactive Analysis) based on tumor and normal samples from the TCGA and the GTEx databases (accessed March 2025). ( A ) Comparison of TFRC expression in high-grade gliomas (GBM) vs. low-grade glioma (LGG) (n indicated on each plot). ( B ) TFRC expression stratified by canonical GBM molecular subtype (classical, mesenchymal, neural, proneural). Boxes represent the interquartile range, horizontal lines the median, whiskers extend to 1.5×IQR, and individual data points are overlaid. Brackets with asterisks denote statistically significant pairwise differences (see Methods for statistical test). ( C ) TfR1 expression levels in GBM cell lines and HEK293 (non-tumor control). Data taken and adapted from the Human Protein Atlas. ( D ) Representative flow-cytometry histograms of surface TfR1 staining in U87MG, T98G, MO59K and HEK293 cells. Traces correspond to unstained control (red), secondary-only control (anti-mouse IgG–AF647; Orange) and specific anti-CD71 primary staining followed by anti-mouse-AF647 secondary (blue). The horizontal bracket on each histogram indicates the gate used to define TfR1-positive events. ( E ) gMFI of the CD71 signal (mean ± SD; n = biological replicates indicated in Methods), and ( F ) percentage of TfR1-positive cells. Data were normalized to appropriate controls. Statistical comparisons were performed as described in Methods (* p < 0.05 ).

Article Snippet: In addition, representative immunohistochemistry (IHC) images illustrating TfR1 protein expression in human brain tissues were retrieved from the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org ), an open-access resource providing antibody-based protein expression profiles across normal and cancer tissues.

Techniques: In Silico, Expressing, Gene Expression, Comparison, Control, Flow Cytometry, Staining